Supplemental material

Sub-classification of response regulators using the surface characteristics of their receiver domains. Kojetin DJ, Thompson RJ, & Cavanagh J.  FEBS Lett (2003) 554, 3: 231-236 [pubmed] [pdf] [corrigendum] [supplemental data]

IMPORTANT NOTE:
Table 3 shown below contains the correct sub-classes -- please refer to it instead of the table shown in the manuscript and the corrigendum.

1. Matrix-based sequence alignment of the regulatory domains from the OmpR subclass of response regulators Bacillus subtilis and Escherichia coli. Data are reported as "identical (*), similar (:), somewhat-similar (.)" as a percentage output by t_coffee in BLAST format. Evaluate mode in t_coffee was set to "slow".


1. Table 1. Sequences from B. subtilis (evaluate_mode = t_coffee_slow)


2. Table 2. Sequences from E. coli (evaluate_mode = t_coffee_slow)


2. Information concerning of sub-classification procedure and an example.


1. Table 3. Data used to aid in the division of regulatory domain-based sub-classes from the OmpR sublclass of response regulators Bacillus subtilis and Escherichia coli.


2. Figure 1. Example (using sub-class A from B. subtilis) of strip positioning, analysis and α1/α5 hydrophobic content totals.

If time allows, a web-based database will be developed that will contain information about the analysis performed in this manuscript.

please address all questions to john_cavanagh@ncsu.edu